The rising trend of plant-based burger in the world market reflects the emerging awareness of ensuring food security while reducing carbon footprints to achieve environmental sustainability. Plant-based meat substitute is introduced to the market to satisfy the demand of those who are willing to reduce the meat consumption but refuse to compromise on the taste of meat. Burger patty mimicking the sensory attributes of beef patty is the primary product of plant-based burger. The purpose of this research is to examine how tasting experience would affect the preferences and willingness to pay by the young generation for plant-based meat substitute burger in Taiwan using discrete choice experiment. The experiment sessions are conducted on campus with the recruitment of undergraduates as participants. Our main results suggest that tasting experience can increase the young people’s willingness to pay for plant-based burger. Young people show stronger preference for the plant-based meat substitute burger after the tasting experiment. Those who have a preference for plant-based meat substitute patty after the tasting experiment are more likely to be females who believe that plant-based meat substitute burger is healthier than beef counterparts. Young people remain to be price sensitive regardless of the tasting experiences. The potential negative effects from higher saturated fat in the food product might be ignored after the tasting, implying the meat substitute patty outweighs other nutritional concerns and play more critical role in determining the change in preferences among food substitutes. Retailers could consider tasting trials as one of their marketing strategies to raise the acceptance of plant-based meat substitutes by the young generation, while keeping the price at the affordable level for young generation across different age groups and occupations. Potential food policy implications can be targeted toward the retailing side to encourage more new customers who might not be interested in such meat substitute but would however alter their behavior when tasting events are available.