Gout is characterized by hyperuriceamia, which is super-saturation of uric acid in the blood. Underexcretion of uric acid, which is the most important in the hyperuriceamia. SLC22A12 and ABCG2 are two major regulators of the uric acid homeostasis. According to previous findings, some sensitive single nucleotide polymorphisms (SNPs) in the SLC22A12 and ABCG2 genes are correlated with the hyperuriceamia and gout in the different populations. However, the genetic study of SLC22A12 and ABCG2 is still unclear in Taiwanese population. Therefore, the aims of this study is to explore the unique sensitive SNPs in the SLC22A12 and ABCG2 genes, which are correlated with hyperuriceamia or gout among Taiwanese population and use the sensitive and low-cost approach, such as SnapShot assay, to detect the sensitive SNPs rapidly for clinical application. In this study, we use the NGS approach to detect normal, hyperuricemia and gout populations. The results of this study indicate the twelve hotspots of SLC22A12 in normal and hyperuricemia or gout population did not show significant differences. On the other hand, rs2231142 and rs4148155 of ABCG2 have significant differences in normal and gout groups (p value ＜0.05, odds ratio and 95%CI are both 3.98 and 2.16~7.32). The specificity of the comparison result in the Snapshot assay and NGS panel is 100%. In conclusion, the clinical application of the present study could be established in the future.