This study is focused on the shear behaviour of dowel-type timber joint composed of domestic wood and self-tapping screw. A series of shear test is intended for evaluating the shear capacity of timber connections. Then, the testing results are compared to the analytic values based on Eurocode 5’ formula. The comparison contributes to verify the feasibility of the formula for domestic materials, including wood and screw. This study selects viable timber and self-tapping screw to manufacture various timber joints. The wood comprises softwood and hardwood for structural purposes, while the self-tapping screw consists of 5 different profiles in terms of screws’ tailing and threading. Depending on these parameters, the shear test includes 16 types of timber joints and each type consists of 9 specimens. The testing results demonstrate that the species of wood influence the shear behaviour significantly. With greater density, the hardwood reveals higher capacity and ductility compared to softwood. Thread’s spacing and the threading length influence joints’ shear behaviour as well. The self-tapping screw with 3mm spacing of the thread increase the ultimate shear strength and ductility by 9% and 7%, respectively, compared to the specimens with 5mm-spacing screw. When applying fully threaded screw, the joints exhibit 9% higher shear capacity and 7% greater ductility than those with half-threaded screws do. According to the comparison between testing and analytic results, Eurocode 5 method conservatively predicts the joints’ capacity. Although the formula may need further modification for Taiwanese materials, the calculation results ensure considerable safety margin for timber joints.