以連續稀釋法評估銀離子抑菌防汙效果

The serial dilution techniques in the fast evaluation of Ag+antifouling performance

從古至今海生物腐蝕的問題一直存在於全球海洋工業中，因此如何尋求因應之道或是找出針對台灣海域所設計的海洋腐蝕抑制材料與檢測技術是非常重要之課題。本研究以銀離子為抗菌物種，搭配選用自左營港採樣所分析出來的代表菌種做為抗菌目標來制定相關流程，以最小抑制濃度（Minimum Inhibitory Concentration, MIC）及最小殺菌濃度（Minimum Bactericidal Concentration, MBC）的概念，分別搭配96孔盤稀釋法與瓊脂稀釋塗佈法進行檢測，設計出短時間提供快速且準確的抗菌劑添加量。研究成果指出在使用金屬銀離子作為殺菌劑時，得到其對於希瓦氏海藻菌的最小殺菌濃度為50ppm。

Biofouling is always one of the major problems in the worldwide marine industries. As a result, it is urgent and necessary to develop the non-toxic antifouling marine agents and techniques for the measurement of antifouling performance, especially in Taiwan marine environment. In this study, an Ag+ antifouling agent was tested. Shewanella algae was chosen as the target bacterial and following the concept of MIC and MBC. The MIC and MBC values of Ag+ concentration will be designed and confirmed by 96-well plate microdilution checkerboard method. It was found that the MBC value of Ag+ against Shewanella algae is 50ppm. With the serial test methods above, the optimal added-amount would high effectively determine.