| 英文摘要 |
DNA barcoding has emerged as a powerful tool for species identification, yet its efficacy can vary significantly across plant taxa. This study evaluates the discriminatory power of five DNA barcode loci—rbcL, matK, trnH-psbA, trnL-F, and ITS (internal transcribed spacer)—recommended by the Consortium for the Barcode of Life (CBOL), using Setaria (Paniceae, Panicoideae, Poaceae) species in Taiwan as a case study. Our results demonstrate that ITS and trnL-F possess the highest discriminatory capacity due to their high sequence variability and PCR success rates. In contrast, trnH–psbA, although easily amplified, provided minimal discriminatory power among species. Core barcodes rbcL and matK showed moderate effectiveness. Notably, some closely related species, such as S. italica, S. viridis, and S. verticillata, could not be reliably distinguished using any single marker. A combined approach using ITS and trnL-F provided markedly improved discriminatory power among species. These findings highlight the importance of taxon-specific marker selection and support the use of multi-locus barcoding strategies for effective plant identification, particularly in taxonomically complex genera like Setaria. |
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