Long-term protective immunity against Vibrio harveyi in grouper induced by single vac-cination with poly（lactide-co-glycolide）microparticles capable of releasing pleurocidin peptide（PLE）and glyceraldehyde-3-phosphate dehydrogenase（GAPDH）

Long-term protective immunity against Vibrio harveyi in grouper induced by single vac-cination with poly（lactide-co-glycolide）microparticles capable of releasing pleurocidin peptide（PLE）and glyceraldehyde-3-phosphate dehydrogenase（GAPDH）

The peptide adjuvant, pleurocidin（PLE）, and the Vibrio harveyi antigen, recombinant glyceraldehyde-3-phosphate dehydrogenase（rGAPDH）protein, were encapsulated with poly（lactide-co-gly-colide）（PLG）polymers in our previous study to produce PLG-encapsulated PLE plus rGAPDH microparticles（PLG-PLE/rGAPDH MPs）that sustained stable release of both PLE and rGAPDH as well as, after two-time vaccination with MPs, generated long-term protective immunity against V. harveyi in grouper.