酶連鎖反應偵測無病徵鰻魚體內之鰻魚疱疹病毒

A Polymerase Chain Reaction for Detecting Herpesvirus anguillae in Asymptomatic Eels

本研究報告一種偵測鰻魚疱疹病毒DNA的聚合酶連鎖反應方法。反應使用的引子（622F和622R）係根據此病毒基因組庫中一段命名為HindⅢ-1550的序列構築而成，以病毒DNA為模板可成功增幅出一段長度符合預期為622鹼基對的片段。本反應具有迅速、靈敏和專一的優點，可由此病毒之台灣分離株與日本分離株中偵測出病毒DNA的存在。利用此反應調查台灣養殖鰻魚之鰻魚疱疹病毒潛伏性感染情況，結果在無病徵魚體內測出病毒持續性感染。

The present study describes a polymerase chain reaction (PCR) assay for detection of Herpesvirus anguillae (HVA) DNA. PCR primers (622F and 622R) were developed based on a HindIII-1550 bp DNA fragment from HVA genomic library. Templates of HVA DNA yielded an evident amplification product showing the expected mobility of a 622-bp DNA fragment. The assay is rapid, sensitive and specifically detects HVA DNA including both the Taiwanese and the Japanese isolates. HVA persistent infections in asymptomatic farmed eels at Taiwan were revealed successfully in the present study.