Ceropegia bulbosa Roxb. var. bulbosa試管中開花與莖節之枝稍繁殖

In Vitro Flowering and Shoot Multiplication from Nodal Explants of Ceropegia bulbosa Roxb. var. bulbosa

本研究提供Ceropegia bulbosa Roxb. var. bulbosa一種快速的微體培養系統。取自溫室栽種植珠之年青健康枝稍的前五節大約各1公分長的莖節，將之培養於B5培養基中，其內含不同濃度之BA與AdS，並均含有0.05 mg/L的NAA。在含有BAP（3mg/L）與NAA（0.05 mg/L）的培養基中，一星期內即可觀察到多至12個再生枝稍，並且在同一培養基的繼代培養中，芽可再增殖。約3-4 cm長之枝稍可在含有2 mg/L之IBA培養基中發根，此發根的小苗可移植於花盆中，成功率約為70%。在BA（0.5 mg/L）與GA3（1 mg/L）的培養基中可觀察到試管中開花。枝稍移植到含kinetin（0.05 mg/L）與IBA（2 mg/L）的培養基中，28天後可觀察到小塊莖，而枝稍培養於GA3（1 mg/L）與BA（0.5 mg/L）的培養基中則有76%的枝稍開花，並可形成種子，其種子萌芽率達65%。

A rapid micropropagation system was developed for Ceropegia bulbosa Roxb. var. bulbosa. First five nodes of ~ 1.0 cm each, harvested from young healthy shoots from garden raised plants were cultured on B5 medium supplemented with different concentrations of BA and AdS each in combination with 0.05 mg/L NAA. Multiple shoot formation of upto 12 shoots was observed within one week in presence of BAP (3 mg/L) and NAA (0.05 mg/L). Shoots were multiplied by subculture on the same medium. Shoots of 3-4 cm length were rooted in medium supplemented with 2 mg/L IBA. The rooted plantlets were hardened and successfully established in pots at 70% success rate. In vitro flowering was observed at 0.5 mg/L BA+1 mg/L GA3. Shoots transferred to the medium containing kinetin (0.05 mg/L) + IBA (2 mg/L) showed microtubers in 28 days while the shoots cultured in presence of 1 mg/L GA3 +0.5 mg/L of BA showed 76% flowering leading to seed production and 65% of the seeds germinated in successive generations