北臺灣煙麴黴（Aspergillus fumigatus）種內變異之分析

Variation in Heat Shock Protein Synthesis and Genomic Diversity in the Natural Population of Aspergillus fumigatus in Northern Taiwan

北臺灣不同區域內煙麴黴（Aspergillus fumigatus Fres）生物歧異度之調查。結果獲得三種生理小種。其形態上的差異反應出對熱逆境有不同之表現。每個品系形成熱休克蛋白質的變異範圍為26-150 kD，其中C 8404-9品系，於40°C，會合成26, 28, 52, 53,87, 150 kD等六種熱休克蛋白質；C 8404-17品系，於40°C，會合成26, 28, 87 kD；而C 8509-3品系，於40°C及50°C，僅合成87 kD。三個品系，均可合成87 kD熱休克蛋白質，故熱休克蛋白質87 kD可認為A. fumigatus化學分類之標記蛋白質。若利用限制酶水解基因片段多形性（RFLPs）方法研究。以限制脢EcoRI和EcoRV水解A. fumigatus總基因（total DNA），分析結果，其相似度範圍為0.67-0.92。此可證實分佈在北台灣煙麴黴（A. fumigatus）之種內歧異度。

Biodiversity of natural population of Aspergillus fumigatus Fres. was investigated from the different localities of northern Taiwan. Three physiotypes of intraspecific diversity of this species were recognized. Accompany to the morphological modification response to thermal stress, each strain of A. fumigatus varied significantly to produce heat-shock proteins (HSPs) ranged from 26 - 150 kD. Strain C 8404-9 synthesized six HSPs of 38, 46, 52, 53, 87 and 150 kD at 4o•c. Strain C 8404-17 synthesized three HSPs of 26, 28 and 87 kD at 40°C. Strain C 8509-3 synthesized only one HSPs of 87 kD at both 40°C and 50°C heat-shock treatments. A HSPs of 87 kD is a common HSPs for A. fumigatus and can be a chemotaxonomic marker of this species. RFLP of total DNA digested with EcoRI and EcoRV could distinguish three physiotypes with the coefficient of similarity ranged from 0.67 to 0.92. It is thus confirmed that there exists intraspecific diversity of A. fumigatus in natural population distributed in northern Taiwan.