銅對百日草葉中原生質膜的脂質、光合作用色素及硫化物含量的影響

Effects of Copper on the Changes of Membrane Lipids, Photosynthetic Pigments and Thiol-containg Compounds in Zinnia elegans Leaves

五週大的百日菊幼苗以含或不含50µm硫酸銅的營養液培養後，以PEG-Dextran雙相分配系純化原生質膜的方法，收集取樣分離與測試。經分析顯示Palmitic acid（16:0）、Linoleic acid（18:2）及Linolenic acid（18:3）為三種主要的脂肪酸，不飽和脂肪酸與飽含脂肪酸的比例因銅處理而減低。銅處理也促進脂質過氧化物的生成和非蛋白質性的含硫化物的合成，同時造成百日草幼葉中葉綠素及總類胡蘿蔔素的增加。

Five-week-old plants of Zinnia elegans were grown in culture medium containing 50 µm CuSO4. Plasma membrane was isolated and purified from both leaves of control and susceptible plants with method of polyethylene glycol/dextrin two-phase partition system. The major fatty acids of plasma membrane were identified as palmitic acid (16 : 0). linoleic acid (18 : 2) and linolenic acid (18 : 3). The ratios of unsaturated fatty acids to saturated fatty acids were reduced in copper-treated plants. The productions of lipid peroxides and nonprotein thiol-containing compound were obviously enhanced in copper-treated plants. Copper also caused the increment in chlorophyll content and total amounts of carotenoids in young leaves.