水稻組織酸性磷酸酶的定位

Localization of Acid Phosphatase in Rice Tissue

臺農67水稻組織經Gomori溶液處理，藉電子顯徵鏡觀察酸性磷酸酶的次細胞分布，癒合組織中酸性磷酸酶分布於細胞、細胞核及液胞中。只於分化培養基14天已綠化之組織，除了前提各胞器外，於綠化期間，大量含有澱粉粒的色素體內，也具有高活性的酸性磷酸酶，在葉中，酸性磷酸酶也出現於粒線體、高爾堤體及細胞質中，而根部分生組織內，分布較廣，如細胞核、細胞壁及粒線體等，在延長部的細胞則主要出現於細胞壁內。

Subcellular localization of acid phosphatases by the method of Gomori showed that they mainly distributed in cell wall, nucleus and vacoule in callus tissue. In greening calli ready to differentiation, acid phosphatases were also found in plastid. In leaf, they were also present in mitochondria, Golgi and cytoplasm. While in root except meristematic tissue, acid phosphatases mainly appeared in cell wall.