電子顯微技術處理之紅合歡（Calliandra haematocephala Hassk.）多粒體花粉生長過程的研究

Studies on the development of polyad of Calliandra haematocephala Hassk. with electron microscopic technique

Development of polyad g rain of Calliandra haematocephala Hassk. was studied. Electron microscopy technique preparation was adapted for this study. That is, pre-fix in glutaraldehyde, post-fix in osmium tetraxoide, dehydrate in acetone, embed in plastics, cut with ultramicrotone, thick section (1µ thick) stained with methylene blue azire II, examine under light microscope. Different developmental stages, namely, undifferentiated, one-, two-, four -, five-, six-, and eight-celled stages were revealed. Each nuclear division is followed immediately by cytokinensis which is common in Monocotyledons and also found in this species. Sporopollenin starts adding on the surface when 4-celled stage is reached (wall formation). Although cell plate has already separated two daughter cells away but sporopolleninous wall had not appeared at that time. Longitudinally, there can be several octads formed from each locule, however, horizontally there is only one expected at the same level. In other words, there was only one pollen mother cell existing at the initiation stage. Accompanying events, such as tapetal layer (development and disintegration), and reinforcement of endothecial cells are also reported.