英文摘要 |
For the past few years, the author has been interested in the developmental aspects of plant biology. The following is a short account of the research done or proposed. 1. What percent of respiratory energy is consumed in biosynthesis of macromolecules in growing cells. The cells isolated from the phloem of the Sycamore Maple (Acer pseudoplatanus) can be easily cultured in the modified White medium. These cells have a generation time of 2 days. They form a friable culture in a liquid suspension. This culture can be handled like a bacterial culture, thus is particularly suitable for biochemical and growth studies. Associated with the growth of plant cells are the biosynthetic processes and respiration. It is interesting to estimate the extent to which the metabolic energy is utilized in biosynthesis of proteins and cell wall polysaccharides, the two classes of macromolecules constituting the bulk of the dry matter in plants. This can be accomplished by simultaneously measuring the respiration and the incorporation of isotopically-labeled precursors. |