化粧品中Salvinorin A檢驗方法之建立   全文下載

Development of the Test Method for Salvinorin A in Cosmetics

Salvinorin A為墨西哥鼠尾草（Salvia divinorum）中致幻覺效果的主要成分，其迷幻效果類似大麻，逐漸受到關注與採取管制措施，依據化粧品衛生安全管理法及相關規定，化粧品成分之使用，應符合相關規範並確保化粧品之品質與保障消費者使用安全。為暸解含鼠尾草成分之化粧品是否符合相關規範並保障消費者權益，本研究目的為建立化粧品中salvinorin A檢驗方法，並進行9件市售產品測試，以評估方法可行性。檢體前處理採用液液萃取，搭配液相層析串聯質譜儀（liquid chromatograph/ tandem mass spectrometer, LC-MS/MS）進行分析，層析部分採用Accucore RP-MS管柱，使用梯度流洗，分析時間共16分鐘。添加回收試驗為於空白樣品（乳液、肥皂及精油）中添加20μg/kg及100μg/kg之標準品，其平均回收率分別介於83.1-103.1%及87.4-108.8%，添加20μg/kg標準品之同日與異日變異係數分別為3.6及7.1%（乳液）、5.4及6.7%（基底油）、3.0及6.5%（肥皂），顯示方法準確度與重複性皆良好，本方法之定量極限為20μg/kg。

Salvinorin A is the main component of the powerful hallucinogenic plant Salvia divinorum. The hallucinatory effect of salvinorin A in Salvia divinorum is similar to marijuana, and its drug abuse has become a new trend and needs more concern. According to the Cosmetic Hygiene and Safety Act and relevant regulations, the ingredients must be safe for consumers under customary conditions of use, companies and individuals who market cosmetics have responsibility to ensure the safety of their products. The aim of this study was to develop a method for the determination of salvinorin A in cosmetics. Nine products were used for evaluating robustness of the method. The liquid-liquid extraction was used for sample preparation. Gradient elution of the mobile phase was carried out through an Accucore RP-MS column with total run time of 16 minutes. The recovery tests were carried out by spiking 20 μg/kg and 100 μg/kg standards into blank samples (lotion, soap and base oil). The recoveries of salvinorin A were between 83.1-103.1% and 87.4-108.8%, respectively, and their intraday and interday coefficients of variation were 3.6 and 7.1% for lotion, 5.4 and 6.7% for base oil, and 3.0 and 6.5% for soap at the spiked level of 20 μg/kg. Both accuracy and precision met the guideline for validation of chemical methods. The limit of quantitation was 20 μg/kg for salvinorin A .