| 中文摘要 |
初級芳香胺（Primary aromatic amines, PAAs）為一類化合物總稱，係偶氮染劑之前驅物。而聚醯胺材質廚房食品用具可能使用偶氮染劑作為染劑或聚合單體，若烹調過程釋出PAAs污染食品，恐產生攝食風險。本研究依據國際法規文獻及參考IARC風險分類，完成27品項PAAs目標物質之分析方法。溶出條件依循我國食品器具容器包裝衛生標準，選擇4%醋酸、去離子水及20%乙醇作為溶出用溶劑，分別進行溶出測試，溶出後經液相層析串聯質譜儀分析。經評估，4%醋酸於95℃加熱30分鐘有較高PAAs溶出量，故選擇以此條件進行後續確效評估。確效試驗部分排除不耐酸或不耐高溫之3品項PAAs進行添加回收試驗，於溶出液中分別添加27品項PAAs標準品，使溶出液含各分析物濃度0.001、0.005及0.01μg/mL，結果顯示平均回收率介於83.4 -124.8%，變異係數介於0.5 - 11.4%，異日添加回收分別添加0.001及0.005μg/mL，變異係數介於0.9 - 19.2%，均符合本署食品化學檢驗方法之確效規範。本方法之溶出液定量極限訂為0.001μg/mL。為驗證本建立方法之適用性，進行13件產品檢測，結果有2件檢出ANL或4,4'-MDA，ANL含量為0.024及0.025μg/mL，4,4'-MDA含量為0.602μg/mL。 |
| 英文摘要 |
Primary aromatic amines, also called PAAs, are a group of chemical compounds that used as precursors for azo-dyes and polyamide, which are made into polyamide kitchenware. Since PAAs are widely used in the kitchenware, it may migrate from kitchenware into food, and people might explore under the risk of food safety hazards. This study aimed at developing an analytical method for the determination of PAAs migrated from polyamide kitchen cooking utensils. Based on the international regulations, research papers and IARC classification, 30 PAAs were selected. The migration conditions followed the regulation of Sanitation Standard for Food Utensils, Containers and Packages (Taiwan), which applied 4% acetic acid, deionized water and 20% ethanol as food simulants for testing. The results showed that the highest amount of PAAs were found migrated from utensils under the condition of 4% acetic acid at 95℃ for 30 minutes. Therefore, 4% acetic acid was chosen as the simulant for method validation. Three PAAs were excluded due to they were unstable under acid and high temperature conditions. The matrices extracted from blank samples by 4% acetic acid were fortified with 27 PAAs compounds at concentrations of 0.001, 0.005 and 0.01 μg/mL (as simulant’s concentration) individually. The average recoveries were between 83.4% and 124.8%, and the coefficients of variation (CV) were between 0.5% and 11.4%. The CVs of interday test were 0.9 - 19.2%. The LOQ of each PAA in this method was 0.001 μg/mL. A total of 13 samples of polyamide ladles and paddles were analyzed by the established method, and the PAAs including ANL and 4,4’-MDA were detected in 2 samples. The concentrations of ANL were from 0.024 to 0.025 μg/mL, and that of 4,4'-MDA was 0.602 μg/mL. |
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