葉綠素螢光在植物生態生理學研究之應用

Applications of Chlorophyll Fluorescence in Plant Eco-physiological Research

葉綠素螢光可作為光合作用研究之有用探針，能反映光合作用的光能吸收、消散及傳遞等訊息，並與光合系統II效率、電子傳遞速率、質子梯度、ATP合成及二氧化碳固定等「生理現象」相關。是植物逆境生理及生態生理學研究上的重要指標，可應用於農業生產、棲地復育及生態監測等方面。近年來，在葉綠素螢光測定之試驗設計，以及螢光參數的進階應用上，均有長足進步，使其在植物生態生理之應用更加廣泛。葉綠素螢光測定技術具有測定簡單、快速、準確性高、非破壞性及儀器輕巧等優點，可在野外現場大量測定，提供各項光生理參數。惟葉綠素螢光牽涉到複雜的生理機制，因此，做好試驗設計及拿捏測定時機是為成功關鍵。

Chlorophyll fluorescence is a useful tool for photosynthesis research. It can reflect the light reaction process of photosynthesis, such as absorption, quenching and transfer, efficiency of photosystem II, electron transport rate, proton gradient, ATP synthesis, and CO2 fixation. Therefore, chlorophyll fluorescence can provide several important indicators for stress- and eco-physiological researches of plants in the fields of agricultural production, habitat construction, and ecological monitoring. In recent years, great progress has been made in experimental design and advanced applications of chlorophyll fluorescence parameters, making it more widely used in the plant eco-physiology field. The chlorophyll fluorescence measurement has the advantages of being simple, fast, reliable, non-destructive, and as a light instrument; it also provides various photo-physiological parameters in large quantities in the field. However, chlorophyll fluorescence involves many complicated physiological mechanisms. Therefore, the experimental design of a research and the timing to determine these parameters are very important.