甘藷蟻象合成性費洛蒙誘蟲活性之風洞檢測

"The wind tunnel assay method for synthetic sex pheromone of sweet potato weevil, Cylas formicarius elegantulus"

在長300 cm、寬77 cm及高75 cm之風洞中，於27.0±2.0℃、70±5% RH、12L:12D光照週期、風速0.17～0.20 m/s及光照度36.7±8.2 lux條件下，探討及檢測甘藷蟻象性費洛蒙誘蟲活性之方法與效果。測試時，於風洞前端上風處75 cm處置放甘藷蟻象玻璃漏斗檢定器，另於距離檢定器放置處150 cm之下風處釋放50-200隻雄蟲，經30 min後觀察記錄誘捕蟲數。於風洞中，20隻甘藷蟻象雌蟲對雄蟲之誘引力表現於晚間，於21:00～24:00達高峰，反應率僅為4.8～5.7%。雄蟲於一天24小時中對其性費洛蒙誘餌均有反應，每小時經30 min之平均反應率為29.5±6.1%。比較不同周齡雄蟲對性費洛蒙之反應，以二、三及四週齡者較佳，反應率分別為40.5、43.0及42.4%；一及五週齡者對性費洛蒙之反應率降低，分別為25.6及32.3%。不同批次合成性費洛蒙對甘藷蟻象之誘蟲活性有差異，雄蟲反應率概在33～44%。不同包裝材質之密封性測試顯示，甘藷蟻象雄蟲對費洛蒙誘餌以PVC塑膠袋包裝者之反應率最高30.1±5.7%；以鋁箔紙及鋁箔袋包裝者反應率低，僅分別為3.4±1.8%及2.0±1.4%；空白組為0.6±0.5%；由此結果顯示甘藷蟻象性費洛蒙誘餌以鋁箔袋與鋁箔紙包裝貯存，密封性佳，而PVC塑膠袋則不適合用來包裝費洛蒙誘餌。

"Assay methods for sex pheromone of sweet potato weevil, Cylas formicarius elegantulus, were studied in a wind tunnel with the specification of 300 cm length, 77 cm width and 75 cm height at 27.0±2.0℃, 70±5 % RH, 12L: 12D photoperiod, 0.17-0.20 m/s wind velocity and light intensity 36.7±8.2 lux. The attractive period of 20-female weevils was found in scotographase. Peak activity occurred at 21:00～24:00, and reactive rates were only between 4.8 to 5.7 % by wind tunnel tests. Whereas male weevils responded to sex pheromone lure in all 24 hrs, and the reactive rate was 29.5±6.1 % in average. Response of different ages of male sweet potato weevils to the synthetic sex pheromone lure in wind tunnel showed that 2, 3, and 4-weeks-old male weevils performed higher response than 1 and 5-weeks-old male weevils. The reactive rates of the former were 40.5, 43.0, 42.4 % and the latter were 25.6, 32.2 %, respectively. Reactive rates of male sweet potato weevil to different batches of synthetic sex pheromone lures in wind tunnel were between 33-44 %. The results of wrapping materials for sex pheromone lures showed that aluminum bag (or paper) was more suitable than plastic bag for wrapping sex pheromone lures of sweet potato weevil."