In January 2020, an epidemic pneumonia caused by the novel coronavirus (COVID-19) broke out in the world. Hemodialysis (HD) is mostly used to treat patients with end-stage renal disease, which require long-term treatment and frequent medical visits. Once a HD patient infected with an emerging infectious disease such as COVID-19, clusters may occur in the dialysis unit if appropriate infection prevention and infection control measures are not taken. To ensure the safety of the medical staff and the patients, and to prevent the occurrence and spread of infectious events, the HD Unit initiated a series of response procedures. Our hemodialysis unit updated and revised the internal regulation and measures in response to the COVID-19 epidemic abiding by the “Guidelines for infection control in medical institutions responding to COVID-19 infection” of the Taiwan Centers for Disease Control (CDC), the “Hemodialysis Room Infection Control & Management Procedures” of the Kaohsiung Medical University Hospital (KMUH), and the “KMUH Staff Pandemic Screening Principles”. Our key procedures include personnel management (focusing on patient triage, patient allocation, and dividing and fixed care group), personnel education (to enhance knowledge and awareness to the COVID-19 pandemic of the medical staffs and the patients), and environmental control (stringent disinfection and sanitization of equipment & machinery are mandatory). We have a good interim result regarding the infection prevention and infection control to the COVID-19 pandemic in Taiwan. We share our HD Unit’s experience and policies during this period. Through mutual learning, cooperation, and teamwork, the medical system can better prepare and response to any future challenges of novel infectious disease.