Recently, Taiwan government has been paying more attention to indoor air quality. Because of the hot and humid climate in Taiwan, large quantities of condensed water are produced in the chilling coils of air-conditioning systems, which could cause significant microbial pollution if an appropriate design and maintenance are not provided. Routine duct cleaning has been suggested as an effective strategy for indoor air quality, and some studies have proposed that applying a coating film to the duct surface can improve microbial pollution conditions. A conference room with an air handling unit was selected for field study in this study. An anti-microbial coating made with nano-zinc was applied to the duct surface after cleaning. Both air sampling and surface sampling were carried out, and the concentrations of bacteria and fungi were calculated using the national environmental analysis laboratory’s method. After 8 weeks of field sampling, the bacterial maintenance factor of the duct surface with anti-microbial coating was maintained at 95%~98%, while the maintenance factor of the duct surface without coating fell to 54%~64%. The results reveal that coating the duct surface with an anti-microbial substance could reduce the frequency of duct cleanliness.