According to the argument of environmental criminology, although the spatial pattern does not directly lead to crime, it has the characteristics of inducing and disguising the criminal behavior. People are able to inspect the environment and detect the dangerous clues. The fear of crime is a sensitive reaction to the dangerous environment. Therefore, this study will explore people’s perception of spatial security in different time periods such as daytime, nighttime and midnight in the aspects of prospect, refuge, escape and illumination, and explore whether the biological sex and psychological gender can bring about any variation in individual’s safety perception. This study takes a university campus as a demonstration base and adopts field survey to make the observers observe the designated place. There are 100 valid samples for each period. The results show: (1) The 3 spatial characteristics of prospect, refuge, and escape all influence safety perception in different time periods, whereas illumination is the most important factor affecting the sense of security in the evening and at midnight (after 23:00). (2) Not only the physiological gender but also psychological gender will affect the sense of security. Those with bisexual and masculine traits have higher sense of security, and feminine persons have the lowest sense of security. (3) The influence of spatial factor on security is greater than individual factor.