In line with the teaching objective of ""inquiry and implementation"" as specified in the 12-year Basic Education, this study applied the PO+E teaching method to train 24 primary pre-service teachers who took the course of ""Primary natural science teaching material and method"" in a National University of Education to develop primary-school nanotechnology lesson plan in conducting micro-teaching and trial teaching. This study used Assessment of PO+E Design teaching plan (APD) to evaluate the nanotechnology plan, and Micro Teaching Assessment of PO+E (MTAP) to evaluate micro-teaching and trial teaching. The results were as follows: first, pre-service teachers could apply the nanotechnology content learned in the ""Science and Technology"" course and successfully convert it into nanotechnology teaching plan. Second, pre-service teachers could apply the PO+E teaching method to conduct micro-teaching and trial teaching. However, teachers should not explain too much content when ""making prediction"", and should use language expressions understandable to primary-school students to develop their ""scientific interpretation ability"". Third, the PO+E teaching method is suitable for the teaching of ""inquiry and implementation"". It is recommended that the pre-service teachers observe senior teachers who used the PO+E teaching method to interact with students in order to enhance learning effects.