Buddhism was introduced to China in the Han Dynasty, and the Buddhist temples have been built in the mainland since then. He-long was one of the earliest areas that Buddhism was introduced to, so the Buddhist temples had been built there in Eastern Han Dynasty. The Two Jins Southern and Northern Dynasties period is an important period in terms of Buddhism development in the Chinese history; simultaneously it is also the most prosperous time for Buddhism in the He-long area. However, there are very few historical records and Buddhist classics about the Buddhist temples in that period in the He-long area. In this paper, the historical records and Dunhuang literature were examined, and 20 Buddhist temples in that period in the He-long area were explored. By investigating the 20 Buddhist temples distributing conditions, certain characteristics of region distribution in that period in the He-long area were obtained. There were no records in the history books and Buddhist scriptures about Buddhist monasteries architectural forms of He-long region in the Two Jins and Southern and Northern Dynasties. However, by analyzing the archaeological results of Buddhist temples in the adjacent Western Region, and the information revealed in the historical books, we can speculate that pagoda-shaped temples were also very popular in the He-long region in this period.