Serosurveillanceof hantavirus antibodies in rodents was conducted at Port of Taichung from 2011 to 2016. A total of 221 rodents were trapped alive and 3 species were identified. Rattus norvegicuswas the dominant species (61.1%), followed bySuncus murinus (26.7%) and Rattus losea (12.2%). All serum samples from rodents were tested for hantavirus antibodies. The average hantavirus seropositive rate in rodents was 5.9% and hantavirus antibodies were only detected in Rattus norvegicustrapped from 5 out of the 20 sites. Theresults suggested that hantavirus was circulating in Rattus norvegicus. We notified stakeholdersnear the 2 sites with higher seropositive ratesto carry out rodent control measures. No human case of hantavirus hemorrhagic fever had been reported at Port of Taichung during the study period. We recommendthat stakeholders eliminate or minimize contact with rodents in order to prevent hantavirus infections.