應用濾紙層析法作紅藍花及其製劑中Carthamin及Safflor Yellow之鑑別

THE APPLICATION OF PAPER CHROMATOGRAPHY TO IDENTIFY SAFFLOR YELLOW AND CARTHAMIN WHICH EXIST IN SAFFLOWER CRUDE PLANTS AND DRUG PREPARATIONS

紅藍花藥材含有紅色色素（Carthamin）及黃色色素（Safflor yellow）等成分。據日本藥局方第十所載「紅花」藥材之鑑別試驗法檢驗含紅藍花製劑時，常因製劑中其他成分之干擾甚難據予確認，為了改進此項缺點，經多次實驗得知，若將檢品溶液直接點滴於濾紙上，再置入展開槽中以適當溶媒予以展開，能獲得較迅速且正確的結果。 There are two pigments - Safflor yellow and Carthamin - exist in SAFFLOWERS, the two colors can be identified easily by the method recorded in J.P.X, the experiment papers have light yellow color on upper (Safflor yellow) and light red color on lower (carthamin), but it is very difficult to identify the two pigments in Chinese drug preparations with this method. The aim of our study is to find a easy, useful and accurate method to identify the two colors in crude plants and Chinese drug preparations too. Finally we find the Paper Chromatography and Thin Layer Chromatography with choiced solvent are very good to identify Cartham in and Safflor yellow in Chinese drug preparations.