阿里山根節蘭根菌結合體之微細構造及內生菌

Ultrastructure and Endophytes of Root-Fungus Association of 'Calanthe arisanensis'

阿里山根節蘭為臺灣特有種植物；本研究所採之樣本在根菌結合體中所誘導純化之4株內生菌，經分子生物鑑定分別應屬於Sordariomycetes、”Neonectria”和”Xylaria”菌株。另於根菌結合體可觀察到腫大及其表面有稠密的菌絲覆蓋；利用電子顯微鏡進行微細構造觀察，發現在內皮層有菌絲團及老化的菌絲團。因此，本研究結果證實，南投縣郡大林道之阿里山根節蘭之根菌結合體具有蘭花菌根之構造。

The objectives of this study were to investigate the root-fungus association characteristics of ”Calanthe arisanensis”, an endemic species in Taiwan. The isolated fungal strains from ”Calanthe arisanensis” have been identified as Sordariomycetes, ”Neonectria” and ”Xylaria” by a DNA sequence analysis. The root-fungi associations from the site were found swollen and densely adhered to the surface by hyphae under a stereomicroscopy. Young and old peloton structures in the root cortex of ”Calanthe arisanensis” were observed with a scanning electron microscope. These results demonstrate that ”Calanthe arisanensis” could form orchid mycorrhiza in the field.