Cytokinin處理及培殖體截段方式對誘導濕地松（Pinus elliottii Engelm.）側芽生長之效應

The Effects of Cytokinin Treatments and Explant Segmentation on Slash Pine (Pinus elliottii Engelm.) Axillary Bud Induction and Growth

取濕地松60~80 天苗齡之實生苗作為培殖體，使用cytokinin 以浸泡或添加在培養基中的方式誘導其側芽抽長進行微體繁殖。不論採用的是何種誘導方式，培殖體維持全株完整、不要截成多段比較能產生多量的芽體。以短暫浸泡作為誘導處理時，6-benzylaminopurine（BAP）和kinetin（KT）彼此間無法區分出何者較佳。但是高濃度BAP（100 mg/l）會抑制芽體抽長。在GD-1 培養基中添加1mg/l BAP 作為誘導側芽生長之環境時，以處理30~45 天即能達到最佳之誘導效應。在本研究中芽體產出情形最佳的處理，為不截段的培殖體以1.0 mg/l BAP、添加在培養基中的方式誘導四週，再移往含活性碳的培養基中四週。平均一株實生苗在經過八週的誘導與繼代培養之後，可產生3.31 個大芽（長度> 3.0 mm），4.19 個小芽（長度≦3.0mm）。 Slash pine seedlings grown 60-80 days after germination were collected as explants in this study. Cytokinin application was carried either by temporary immersion or by medium addition in order to stimulate the axillary bud elongation. No matter what kind of stimulation the seedling explants received, they would produce more axillary bud from intact or less segmented specimens. 6- benzylaminopurine(BAP) and kinetin(KT) were equally effective to induce bud production when using immersion method. But BAP at a higher concentration (100 mg/l) inhibited bud elongation. When using medium addition method (BAP 1 mg/l in GD-1 medium), the better treatment duration was determined at about 30-45 days. We have had a best bud induction treatment in this study that a seedling explant would well response to the BAP (1.0 mg/l) treatment through a 4-week medium addition method, and then cultured with charcoal (minus BAP) for 4 more weeks. This procedure produced 3.31 elongated large buds (> 3.0 mm) and 4.19 non-elongated small buds (≦ 3.0 mm) per explant.

Slash pine seedlings grown 60-80 days after germination were collected as explants in this study. Cytokinin application was carried either by temporary immersion or by medium addition in order to stimulate the axillary bud elongation. No matter what kind of stimulation the seedling explants received, they would produce more axillary bud from intact or less segmented specimens. 6- benzylaminopurine(BAP) and kinetin(KT) were equally effective to induce bud production when using immersion method. But BAP at a higher concentration (100 mg/l) inhibited bud elongation. When using medium addition method (BAP 1 mg/l in GD-1 medium), the better treatment duration was determined at about 30-45 days. We have had a best bud induction treatment in this study that a seedling explant would well response to the BAP (1.0 mg/l) treatment through a 4-week medium addition method, and then cultured with charcoal (minus BAP) for 4 more weeks. This procedure produced 3.31 elongated large buds (> 3.0 mm) and 4.19 non-elongated small buds (≦ 3.0 mm) per explant.