應用 HPLC、ITS、顯微形態及抗氧化活性方法研究兩種黃耆（膜莢黃耆及多序岩黃耆）   全文下載

Investigation of Two Species of Huang-qi (Aatragalus membranaceus and Hedysarum polybotrys) by HPLC, ITS, Microscopic Morphology and Antioxidant Activities

晉耆做為黃耆的替代品在台灣由來已久。本文採用ITS（internal tran-scribed spacer）顯微鑑定，HPLC指紋圖譜以及抗氧化活性測定等方法對這兩個品種進行鑑別區分。利用上述方法對市場購得的8個黃耆及晉耆樣品進行了分析而獲得了一致性的結果，確認樣品2，3，8為黃耆，而樣品1， 4，5，6，7為晉耆。實驗結果顯示無論是ITS 、顯微鑑別或HPLC指紋圖譜方法均可對黃耆與晉耆進行有效區分。 70%乙醇部份純化物抗氧化活性顯示，晉耆優於黃耆。

Hedysarum polybotrys is commonly substituted for Astragalus membranaceus in Taiwan for many years. This study described several different ways, including microscopic morphology, ITS (internal transcribed spacer) sequencing and HPLC fingerprint methods to identify these two species. Furthermore, the antioxidant activities of the two plants were evaluated and H. polybotrys had higher antioxidant activity than A. membranaceus. All the methods gave consistent conclusions that test samples KRA2, 3 and 8 belonged to A. membranaceus, while KRA1, 4, 5, 6 and 7 belonged to H. polybotrys. The results revealed that all the above methods may successfully discriminate between A. membranaceus and H. polybotrys.