應用ITS序列鑑定茵陳蒿藥材及其製劑   全文下載

Identification of Artemisiae Scopariae Herba and its Preparations Based on ITS Sequence by Nested PCR-DNA Sequencing Method

茵陳蒿藥材基原為菊科植物濱蒿（Artemisia scoparia）或茵陳蒿（Artemisia capillaris）之乾燥地上部分。然而因為外觀型態相近，茵陳蒿與青蒿藥材（原植物黃花蒿Artemisia annua）容易彼此誤用；而牛至（Origanum vulgaris L.）藥材則因為別名為北茵陳、土茵陳，市面上也容易出現同名異物的混淆情況。為避免藥材使用錯誤而影響療效，本研究利用internal transcribed spacer （ITS）序列差異性，設計特異性的引子開發PCR-DNA定序之檢驗方法。價購市面上茵陳蒿及其常見之誤用品藥材總計4種品項36件藥材及38件製劑檢體進行試驗，研究結果證明此方法確實可有效地鑑定茵陳蒿及其誤用品之藥材基原，亦可應用於複方製劑之檢驗。

Artemisiae scopariae herba is the dried aerial part of Artemisia scoparia Waldst. or Artemisia capillaris Thunb. However, it is often confused with other Artemisia species because of their similar outward appearances. It is also confused with Origanum vulgare L. because the latter has similar common names. To prevent the misuse of the herb, specific primers for nested-PCR were designed based on their internal transcribed spacer (ITS) sequences, and amplified products were sequenced for the authentication. In this study, 36 samples of raw herbs and 38 traditional Chinese medicine (TCM) preparations of artemisiae scopariae herba were analysed. The results showed that the nested PCR-DNA sequencing method was useful for the identification of artemisiae scopariae herba in the raw material form and in TCM preparations.