ANALYSIS OF SYNTHETIC GASTROINTESTINAL DRUGS IN ADULTERATED TRADITIONAL CHINESE MEDICINES BY HIGH­ PERFORMANCE CAPILLARY ELECTROPHORESIS   全文下載

ANALYSIS OF SYNTHETIC GASTROINTESTINAL DRUGS IN ADULTERATED TRADITIONAL CHINESE MEDICINES BY HIGH­ PERFORMANCE CAPILLARY ELECTROPHORESIS

Six synthetic gastrointestinal drugs, cirnetidine, hornatropine, rnetoclopramide, pirenzepine, ranitidine and scopolamine butylbrornide, which can be found as adulterants in traditional Chinese medicines were assayed simultaneously using highperformance capillary electrophoresis within 6 min. The electrolyte ws a buffer solution containing 100 mM phosphate buffer (NaH2P04'H3P04, pH 2.5) and 5 %acetonitrile. Applied voltage was 20.5 kV and temperature was 30℃ 3-Benzyl-5-(2-hydroxyethyl) -4-rnethylthiazoliurn chloride was used as m internal standard and detector set at 214 nm. Regression equations revealed linear relationships (correlation coefficients: 0.9990-0.9997) between the peak-area rations of each drug to ethylparaben. The effects of buffer pH and acetonitrile concentration on separation are also discussed. The relative standard deviations of these gastrointestinal drugs for intraday and interday analyses were 0.78-2.15 % and 1.10-2.75 %, respectively . The recoveries of the synthetic drug adulterants in traditional Chinese medicinal formula ranged from 97.3 to102.4 %.