高效液相層析法鑑別六種BENZODIAZEPlNE類安眠鎮靜劑

IDENTIFICATION OF SIX BENZODIAZEPINES IN PHARMACEUTICALS AND ORANGE JUICE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

應用高效液相層析法分離、鑑別不明製劑及摻加於飲料中之Benzodiazepine 類安眠、鎮靜劑：Alprazolam, Bromazepam, Flunitrazepam, Flurazepam HCl, Nordazepam，與 Triazolam。不明製劑以甲醇萃取，摻藥果汁則須於鹼性下以氯仿萃取，萃取液蒸乾後轉溶於甲醇，再以高效液相層析法分析，其條件如下：Lichrospher 100 RP-8 層析管（125mm x 4 mm I.D., 5μm），移動相為甲醇：乙腈（3:2）-0.03M 磷酸二氫鉀緩衝液（pH4.7）（38:62），流速1 ml/min.，偵測波長254 nm，層析時間約30分鐘。本法之最低偵測量為：Alprazolam 7.2 ng, Bromazepam 84.4 ng, Flunitrazepam 3.6 ng, Flurazepam HCl 11.4 ng, Nordazepam 5.2 ng, Triazolam 12.0 ng。

A high-performance liquid chromatographic procedure was used for the separation and identification of Benzodiagepines. The six Benzodiazepines found in unknown pharmaceuticals and orange juice consists of alprazolam, bromazepam, flunitrazepam, flurazepam HCI, nordazepam and triazolam. In this procedure, the methanolic sample solutions were chromatographed on a C 18 column using mobile phase containing methanol, acetonitrile and 0.03 M potassium dihydrogen phosphate buffer (pH 4.7) and detected at 254 nm. The limit of detection of the method is 7.2 ng for aprazolam, 84.4 ng for bromazepam, 3.6 ng for flunitrazepam, 11.4 ng for flurazepam HCI, 5.2 ng for nordazepam and 12.0 ng for triazolam.