三段式接觸生物反應槽處理酚廢水之生物分解特性

Performance of Three Stage Contact Bioreactor for Biodegradation of Phenol

本研究以接觸曝氣法連續進料人工合成廢水及空氣進行酚廢水之處理，在三段式接觸生物反應槽載體區懸吊高孔隙密度結構的石英製生物環，並選擇來自酚醛樹脂製造廠(Syndyne Industry Company)廢水收集站污泥及四周土壤篩選出的耐酚菌種M1及M5等馴養出生物膜（兩種菌株經16S rDNA定序比對，Ml為Microbacterium arborescens相似度96%，MS為Bacillus cereus相似度98%），在不同的操作條件下，進行模型廠操作實驗，以探討酚廢水處理的生物分解特性。結果顯示，系統在水力停留時間一定下，接觸生物反應槽的表面積我負荷越大，酚的轉化率及COD的去除率越差：相反的，表面積負荷越小，酚的轉化率及COD的去除率越佳，尤其是COD面積負荷（gCOD/平方公尺-d）低於5.49，三段系統COD的去除率可達90%去除率，酚面積負荷（g酚／平方公尺-d）低於2.14，酚的轉化率可達100%。本研究系統經10個試程，在穩定狀態下，生物反應速率常數值k (20℃)介於0.027hr^(-1)，與0279hr^(-1)之間。常實際處理流量Q、進流水質濃度Co、要求的放流水質濃度Ce等已知時，可由以下以COD為基準的數學模式求得生物環接觸材料所需的表面積Ac。

This research focused on the treatment of phenolic wastewater by the contact aeration process, which was fed with synthetic phenolic wastewater and air continuously. Quartzite glass cylinders with high-density structure were used as the biofilm carrier, and were hanged and linked to form a bunch of carrier zone m the contact bioreactor. The selected test strains (M1 and M5) were selected from the mixing sludge around of the reactor and wastewater storage in the Syndyne Industry Company (Tainan, Taiwan). The Mi was 96% similar to Microbacteriun arborescens and The MS was 96% similar to Bacillus cereus based on the sequence analysis of 16S rDNA. The effect of operating conditions on the degradation of phenol by test strains in the bioreactor was experimentally studied under various operation conditions. The result was found that the higher surface loading decreased the phenol and COD removal at the steady hydraulic retention time. It can be seen that more than 90% removal of phenol was performed in the surface COD loading beyond 5.49 gCOD/m^2-d. 100% conversion rate of phenol can reach was also found in the phenol surface loading lower than 2.14 g phenol/m^2-d. The biological reaction rate constant value k (20℃) was ranged from 0.027 hr^(-1) to 0.279 hr^(-1) under the stable status by various 10 runs. The needed surface area of biofilm carrier (Ac) could he calculated with the parameters of wastewater discharge Q, influent water quality C0 and demand of effluent water quality Ce. The modified formula was shown as following: (the equation is abbreviated).