南台灣的口腔腫瘤樣本中第一型基質金屬蛋白酶之普遍表現型態

The Universal Expression Pattern of the Matrix Metalloproteinase-1 in Oral Tumor Specimens from Southern Taiwan

本研究的目的乃是要探討在南台灣的口腔腫瘤病人中，與腫瘤有關的第一型基質金屬蛋白酶所扮演的角色。取三十九組手術切除的口腔腫瘤樣本與其週邊組織進行第一型基質金屬蛋白酶與甘油醛-3-磷酸去氫酶兩種基因的半定量反轉錄作用－聚合酶連鎖反應。在所有腫瘤樣本中，均有表現第一型基質金屬蛋白酶（100%），且在這三十九組樣本中，第一型基質金屬蛋白酶基因在口腔腫瘤樣本中的mRNA表現量顯著高於在其週邊組織的mRNA表現量。此外，在上述三十九組樣本中，取其中五組進行西方墨點法，以偵測第一型基質金屬蛋白酶的蛋白質表現情形。結果發現第一型基質金屬蛋白酶在口腔腫瘤樣本中的蛋白質表現量顯著高於在其週邊組織的蛋白質表現量。由此結論第一型基質金屬蛋白酶在腫瘤組織中普遍有表現，且集中表現於腫瘤組織中，因此第一型基質金屬蛋白酶在口腔癌整個發展過程中應扮演重要的角色。

The aim of this study was to evaluate the roles of tumor-associated matrix metalloproteinase-1 （MMP-1） in oral tumor patients from southern Taiwan. Thirty-nine surgical specimens of oral tumors （Ts） and their neighboring tissues （Ns） were subjected to the semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） with the primers of MMP-1 and glyceraldehyde 3-phosphate dehydrogenase （GAPDH） genes. All of the 39 specimen pairs showed preferential expression patterns of MMP-1 messages in Ts than in Ns （100%）. Furthermore, 5 pairs of them, originally stored with more than two pieces of Ts and Ns, were available for further homogenate preparation and MMP-1 protein detection by Western blotting. The results demonstrated that MMP-1 proteins were also predominantly expressed in Ts than in Ns in all the 5 specimens tested. We conclude that MMP-1 may be universally and preferentially expressed within tumor masses and plays important roles throughout the entire developmental process of oral cancers.