以逢機複製多態性DNA (RAPD)技術探討蓮霧大果品系間的親源關係

Genetic Relationships of the Big-fruited Wax Apple-lines Collected from Taiwan by Random Amplified Polymorphic DNA (RAPD)

蓮霧大果品系是由｀粉紅＇品種突變而來，因此以外部型態來分辨大果品系並不容易。本研究以逢機複製多態性DNA (RAPD)技術來探討蓮霧大果品系間的親源關係。在以100 條逢機引子來分析｀粉紅＇和12 個大果品系後，只有8 條引子具有多態性。13 個品系在遺傳相似度係數95% 下可以分成 7 群。｀英盛1＇,自己獨立成1 群。其它 12 個品系可以分成6 群，分別是｀粉紅＇; ｀順德1＇、｀順德2＇、｀順德3＇和｀建智1＇; ｀鳳試2＇、｀鳳試3＇和｀鳳試4＇; ｀鳳試1＇和｀鳳試7＇; ｀鳳試6＇; ｀英盛2＇。

Random amplified polymorphic DNA (RAPD) analysis was used to study the genetic relationships of the 13 big-fruited wax apple (Syzygium samarangense Merr. et Perry) lines whose morphological identification were difficult. Among the 100 primers for the RAPD analysis, 8 primers revealed reproducibly distinct RAPD profiles. The 13 wax apple lines in the 95% similarity can be classified into 7 groups, ‘YingSheng 1’, an independent group, is different from the other 12 wax apple lines with average similarity of only 72%. The other 6 groups are ‘Pink’; ‘SoonDer 1’, ‘SoonDer 2’, ‘SoonDer 3’, and ‘JanGee 1’; ‘FengShe 2’, ‘FengShe 3’, and ‘FengShe 4’; ‘FengShe 1’, and ‘FengShe 7’; ‘FengShe 6’; ‘YingSheng 2’.