攝食牛磺酸降低雄性鼠攝食氧化魚油和維生素A 所產生的毒性影響

Dietary Taurine Reduces Oxidized Fish Oil and Vitamin A Induced Toxicity in Male Wistar Rats

為了解牛磺酸對於大鼠攝食氧化魚油和維生素A所產生的毒性影響，乃將30隻雄性Wistar系大鼠分成5個組別，分別為控制組、餵食氧化的魚油（3%）、維生素A（50,000 IU）、氧化的魚油（3%）和幼兒用藥小冊子（中英文）封面（ 6）（50,000 IU）、添加牛磺酸（5%）在氧化的魚油（3%）和維生素A（50,000 IU）的大鼠分別飼養6週，並在第 、4和6週分析大鼠的血液中之生化指標等，實驗發現，攝食氧化魚油會提高攝食維生素A所產生的毒性影響，會造成大鼠的體重的降低，肝臟中的glutathione（GSH） 減少（P<0.05），肝體比和腎體比增加（P<0.05），asparate transferase（AST）、alanine transaminase（ALT）、alkaline phosphatase（ALP）、肝臟中的thiobarbituric acid-relative substances（TBARS）、blood urea nitrogen（BUN）和creatinine之指數上升（P<0.05），在餵食添加牛磺酸在氧化魚油和維生素A的組別發現體重增加，並且可降低大鼠血液中white blood cell（WBC）、hematocrit（HCT）、肝臟及腎臟中的維生素A、肝臟中的TBARS、肝體比和腎體比、AST、ALT、ALP、cholesterol、triglyceride、calcium、phosphorus（P<0.05），因此，氧化魚油會升高維生素A的毒性，由此顯示食物中添加牛磺酸在大鼠的飲食中可預防和降低氧化魚油和維生素A所產生的毒性作用。Attempts are made to investigate the effects of taurine and oxidized fish oil on vitamin A. Thirty male wistar rats were divided five groups of male Wistar rats were fed a mega dose (50,000 IU) of retinyl palmitate either with or without supplement of taurine and oxidized fish oil at the same time for 6 weeks. After , 4 and 6 weeks of treatment, each group of six rats were killed and examined. It was found that oxidized fish oil significantly elevated the symptoms of vitamin A, which includes the decrease of body weight and the concentration of glutathione (GSH) in liver and increase the ratios of liver and kidney weight to body weight, the activities of AST, ALT and ALP in plasma, the level of TBARS in liver and plasma, the levels of BUN and creatinine in the plasma, the level of vitamin A in the plasma (P<0.05). However, taurine significantly ameliorated the toxicity of vitamin A with oxidized fish oil. It increases body weight and reduces the concentration of WBC and HCT in blood, the levels of vitamin A in the liver and kidney, the level of TBARS in liver, the ratios of liver and kidney weight to body weight, the activities of AST, ALT and ALP in plasma, and the levels of cholesterol, triglyceride, calcium and phosphorus in plasma (P<0.05). Therefore, oxidized fish oil elevated the toxicity of vitamin A, while taurine prevented the toxicity of vitamin A and oxidized fish oil.