市售紅景天藥材中salidroside和p-tyrosol之含量分析

QUANTIATIVE ANALYSIS OF SALIDROSIDE AND p-TYROSOL IN RHIZOMA RHODIOLA

"紅景天為多年生草本或亞灌木植物，為亞洲與東歐常用之藥材，本研究以高效液相層析儀（HPLC ）探討市售紅景天中活性成分salidroside 及p-tyrosol 之含量，結果顯示salidroside 及ptyrosol 之標準曲線分別於50∼400 μg/mL 及25∼400 μg/mL 的濃度範圍內，呈現良好之線性關係（r = 0.9998 ）。同日間（intra-day ）與異日間（inter-day ）試驗之相對標準偏差各為0.35∼3.13% 與0.09∼1.79% ；回收率平均值各為101.5% 與97.5% 。在十二種市售藥材檢品中，每克藥材之salidroside 含量介於1.78∼18.63 mg 之間；而p-tyrosol 含量介於0.28∼7.56 mg 之間。Rhizoma Rhodiola, a perennial herbaceous plant, is a traditional medicine mainly used in Asia and Eastern Europe. Salidroside and p-tyrosol are bioactive constituents of Rhizoma Rhodiola. The goal of this study was to develop an HPLC method for determining salidroside and p-tyrosol in Rhizoma Rhodiola. The results showed good linear relationships between the corresponding peak area and concentration. The linear calibration range of salidroside and ptyrosol were 50~400 μg/mL (r = 0.9998) and 25~400 μg/mL (r = 0.9998), respectively. The relative standard deviation of marker substances for intraday and interday analyses were 0.35~3.13% and 0.09~1.79%, and the average recovery rates of the salidroside and p-tyrosol were 101.5% and 97.5%, respectively. The salidroside and p-tyrosol contents ranged between 1.78~18.63 and 0.28~7.56 mg/g for twelve raw material samples of Rhizoma Rhodiola. "