一氧化氮與神經退化性疾病之關聯性與天然物對一氧化氮產生之影響

Pathogenesis of Neurodegenerative Diseases and the Effect of Natural Products on Nitric Oxide Production Implicating in These Diseases

利用活性追蹤的分離，自樟芝（Antrodia camphorota, strain CCRC-35396 ）的培養液中得到一個具抗B 型肝炎病毒活性之pyrroledione 類化合物，利用光譜分析確認其結構為3-isobutyl-4-{4-[（3-methyl-2-butenyl）oxy]phenyl}-2,5-dihydro-1H-2,5-pyrroledione （1） 。化合物1 在50 μM 時不產生細胞毒活性，但對B 型肝炎病毒之HBsAg 及HBeAg 的表現分別有35.2 及12.8% 的抑制作用。Bioassay-guided fractionation resulted in the isolation of an anti-HBV pyrroledione, 3-isobutyl- 4-{4-[(3-methyl-2-butenyl)oxy]phenyl}-1H-pyrrole-2,5-dione (1), from the culture broth of fungus Antrodia camphorota (strain CCRC-35396). The structure of compound 1 was determined by spectral analyses. Compound 1 suppressed both HBsAg and HBeAg expression with the moderate inhibition percentages of 35.2 and 12.8%, respectively, at the non-cytotoxic concentration of 50 μM.