中草藥對於促發炎細胞激素生成之影響

A STUDY OF THE EFFECTS OF NINE CHINESE HERBS ON PROINFLAMMATORY CYTOKINES PRODUCTION IN TWO CELL CULTURE MODELS

本研究主要以小鼠巨噬細胞株J774A.1 及初代腹腔細胞為實驗模式，探討九種中草藥萃取物對脂多醣類lipopolysaccharide （LPS ）所誘發之促發炎細胞激素TNF-α 、IL-6 及IL-1β 生成的影響。首先以MTS 分析法進行不同濃度中草藥萃取物在LPS 存在下對小鼠巨噬細胞株J774A.1 及初代腹腔細胞存活率之影響，並將收集的細胞培養液進行TNF-α 、IL-6 及IL-1β 之分析。結果顯示：金銀花與雷公根之水萃取物以及五味子、金銀花與杜仲之乙醇萃取物均可抑制由LPS 誘導J774A.1 細胞株生成IL-6 之作用；甘草及杜仲之乙醇萃取物對初代腹腔細胞有明顯抑制IL-6 之生成；杜仲、陳皮及五味子之乙醇萃取物則對LPS 誘導的初代腹腔細胞生成的IL-1β 有明顯的抑制作用。另外，陳皮之水及乙醇萃取物在兩種細胞培養模式下均有抑制TNF-α 生成之作用。Nine Chinese herbal extracts were used to study the inhibition of the proinflammatory cytokines including TNF-α, IL-6 and IL-1β in J774A.1 mouse macrophage cell line and primary peritoneal macrophage cells stimulated by LPS, respectively. All the macrophage cells were incubated with different doses of Chinese herbal extracts and LPS for 72 hours, and cell viability was measured by MTS assay. The supernatants were harvested and the production of TNF-α, IL-6, and IL-1β were measured by the enzyme-linked immunosorbent assay (ELISA). The result showed that water extracts of Lonicerae Flos and Centellae Herba and the ethanolic extracts of Schizandrae Fructus, Lonicerae Flos and Eucommiae Cortex significantly inhibited the production of IL-6 in J774A.1 cells. Among these herbs, Glycyrrhizae Radix and Eucommiae Cortex showed a significant inhibition on the production of IL-6 in LPS-induced primary peritoneal macrophage cells. Inhibition of IL-1β production in LPS-induced primary peritoneal macrophage cells were also observed in the ethanolic extracts of Eucommiae Cortex, Citri Leiocarpae Exocarpium and Schizandrae Fructus. In addition, the water and ethanolic extracts of Citri Leiocarpae Exocarpium also inhibited LPS-induced TNF-α secretion in J774A.1 cells and primary peritoneal macrophages.