龍葵水粗抽物對人類大腸癌細胞胺基乙醯轉移酵素活性的影響

THE EFFECTS OF SOLANUM NIGRUM ON N-ACETYLTRANSFERASE ACTIVITY IN HUMAN COLON CANCER CELL LINES

大腸癌至今仍是台灣地區癌病死亡主要原因之一。龍葵是一種被用來治療癌症的中草藥植物，而龍葵對於人類大腸癌細胞的影響，其作用機轉並沒有確切可引用的報告。在這個研究，我們以乙醯輔酵素A（Acetyl-Co A）再製液及利用高效液相層析儀（HPLC）來分析2-aminofluorene（2-AF）及p-aminobenzoic acid（PABA）的胺基乙醯化的量，檢測龍葵的水粗抽物對人類大腸癌細胞（colo205）胞質液及完整細胞中2-AF 及PABA 的胺基乙醯化的劑量效應和時間效應。結果顯示龍葵水粗抽物可降低人類大腸癌細胞（colo205）胞質液及完整細胞中的胺基乙醯轉移酵素的活性。龍葵治療惡性腫瘤的途徑，以人類大腸癌細胞（colo205）為例子，可能是由於龍葵能降低人類大腸癌細胞（colo205）NAT 活性。Colon cancer is one of the leading causes of death in Taiwan and Solanum nigrum is one of the medicinal plants used in cancer therapy in hospital or just a folk remedy. However there is no available information to address the mechanism by which Solanum nigrum affects human colon cancer cells. We examined the dose effect and time course effect of crude Solanum nigrum extract on the degree of N-acetylation of 2-aminofluorene (2-AF) and p-amino benzoic acid (PABA) in colo205 cell cytosol and intact cell cultures by using acetyl Co-A recycling mixture assay and high pressure liquid chromatography (HPLC). The results revealed that the crude extract of Solanum nigrum reduced NAT activity in colo205 cell cytosol and intact cell cultures. Therefore the effe-ct of Solanum nigrum to treat malignant diseases may be due to its inhibitory effect on NAT activity in colo205 cells.