龍葵水粗抽物對人類胃癌細胞株（SC-M1）NAT1基因表現的影響

THE EFFECT OF CRUDE EXTRACT OF SOLANUM NIGRUM ON THE NAT1 GENE EXPRESSION IN HUMAN STOMACH CANCER CELL LINE (SC-M1)

胃癌至今仍是台灣地區癌病死亡原因之一。龍葵（Solanum nigrum ）是一種被用來治療癌症的中草藥植物，而龍葵對於人類胃癌細胞的影響，其作用機轉並沒有確切可引用的報告。因此在這個研究，我們利用RT-PCR （Reverse Transcriptase Polymerase Chain Reaction ）的方法來檢測不同濃度的龍葵水粗抽物對人類胃癌細胞株（SC-M1 ）NAT1 基因表現的影響。結果顯示龍葵水粗抽物並不會造成NAT1 基因表現（NAT1 mRNA ）的變化。Gastric cancer is one of the causes of death in Taiwan up to now. Solanum nigrum is one of the Chinese herb medicine plants had been used to do anti-cancer. There is no available information to address the mechanism of Solanum nigrum affect human stomach cancer cell. Therefore, in this study, we will detect the effects of the NAT1 gene expression (NAT1 mRNA) of SC-M1 cells by reverse transcriptase polymerase chain reaction (RT-PCR). The result showed that The crude extraction of Solanum nigrum did not reduce the NAT1 gene expression.