白英細胞在Indole-3-butyric acid處理下脂多醣体的定性分析

STUDIES OF LIPOPOLYSACCHARIDES OF IBA TREATED SOLANUM LYRATUM CELL SUSPENSION CULTURE

以hot phenol / water 法萃取白英細胞脂多醣体，經分子篩色層分析純化。在銀染聚丙稀銨不連續電泳，可見到似階梯狀的電泳條帶分佈。從白英細胞經Indole-3-butyric acid 1, 5, 10, 20 mg L-1處理後第21 天萃取出的脂多醣体，由分子篩色層分析得知，高濃度的Indole-3-butyric acid （10, 20mg L-1） 其脂多醣体含醣量高於低濃度的Indole-3-butyric acid 處理下細胞所合成脂多醣体的含醣量。由電泳結果顯示，低濃度的Indole-3-butyric acid （1, 5 mg L-1）處理下細胞只合成核心寡醣及一個單位的O-抗原因子。高濃度的Indole-3-butyric acid （10, 20 mg L-1）細胞能合成不同單位數的多醣鏈接到O-抗原因子上，而形成分子量高的巨型分子。Lipopolysaccharides of Solanum lyratum were purified through hot phenol / water method and gel filtration chromatography. The characteristics of lipopolysaccharides are the ladder –like patterns shown on the SDS -polyacrylamide gel electrophoresis. Comparisons were made between Indole -3- butyric acid (IBA) 1, 5, 10, 20 mg L-1 treated cells for 21 days. The gel filtration profiles showed that high concentrations of IBA (10 and 20 mg L-1), the sugar contents of lipopolysaccharides were higher than that of the low concentrations of IBA (1 and 5 mg L-1) treated cells. Only core polysaccharides and one copy of O- antigen polysaccharides were synthesized for low concentrations of IBA (1 and 5 mg L-1) treated cells. Cells synthesized various repeat units of polysaccharides on the O- antigen side chain under high concentrations of IBA (10, and 20 mg L-1) treatment.