溫病條辨中焦濕溫疸證處方治療ANIT誘發大白鼠膽小管性肝炎的效果

EFFECTS OF CENTER BURNER DAMP WARMTH JAUNDICE PATTERN PRESCRIPTIONS FROM THE WEN-BING-TIAO-BIANIN THE TREATMENT OF ANIT -INDUCED CHOLANGIOLITIC HEPATITIS IN RATS

"本研究目的樣探討溫病條辨中焦濕溫疸證處方，對ANIT 誘發大白鼠膽小管性肝炎的治療效果。實驗分成六組，每組8隻，A組為正常控制組，Ｂ組為ANIT 損傷組，C至E為治療組，C 組為二金湯，D組為杏仁石膏湯，E 組為連翹赤小豆飲，F組為Silymarin 藥物參考組。實驗結果，所有治療組及藥物參考組對ANIT 膽小管性肝炎的血清轉氯酵素（SGOT 和SGPT）、鹼性磷酸酵素（ALP） 、總膽紅素（TB） 土升變化，除二金湯對ALP 的變化無顯著影響外，均有類著降低作用，其中降低血清GOT 、GPT 值的效呆以達翹赤小豆飲最佳。對ANIT肝損傷所造成的脂質過氧化物（LPO） 含量土升，亦均有顯著降低的作用;而對谷胱甘肽（GSH）含量上升的變化則無影響。以Jonker's 病理半定量分析ANIT 膽小管性肝炎治療後的病理組織變化，結泉頰示杏仁石膏湯能顯著減輕肝細胞發炎、減少細胞核的有絲分裂和抗PMN 的浸潤，連翹赤小豆飲有顯著抗肝細胞壞死的作用，而二金湯抗膽小管炎有明顯的效呆。The purpose of the study was to evaluate how effective formulas from the classic of warm disease, the Wen-Bing-Tiao-Bian, intended to treat center burner damp wannth jaundice are in the treatment of ANIT-induced cholangiolitic hepatitis in rats. The prescriptions contain Er-Jin-Tan (EJT), Xing-Ren-Shi-Gan-Tang (XRSGT) and Lian-Qiao-Chi-Dou-Yin (LQCDY). The experimental animals were divided into 6 groups of each 8 rats. Group A was a normal control and group B was ANIT-induced liver injury. Groups C, D and E were treated with EJT, XRSGT, and LQCDY respectively. Group F was treated with reference drug silymarin. The results demonstrated a significant decrease in the elevated serum biochemical levels of sGOT, sGPT, ALP and total bilirubin (TBI) caused by ANIT-induced cholangiolitic hepatitis in all the treatment and control drug groups. Only EJT had no influence on serum alkaline phosphatase (ALP). Of the prescriptions that decreased the serum levels ofsGOT and sGPT, the therapeutic effect of LQCDY was the most marked. In this study the prescriptions significantly decreased the activity oflipid peroxide (LPO) resulting from the A NIT-induced liver injury, although they did not affect the activity of glutathion (GSH) induced by the ANIT liver damage. As to the pathological changes caused by ANIT-induced cholangiolitic hepatitis after treatment with Jonker's semi-quantitative analysis, the results show that the XRSGT can relieve the liver cell inflammation, and can decrease the cell nucleus mitosis and PMN infiltration. LQCDY can fight liver cell necrosis, and the EJT has a marked effect on anti-cholangiolitis. "