治療肝炎中藥方劑之藥理學研究

Pharmacological Studies on the Effects of Traditional Chinese Prescriptions for Hepatitis in Rats

本研究係探討中國醫藥學院附設醫院肝炎特別門診用於治療肝炎之中藥方劑：H1（只實梔豉湯）、H2（丹梔逍遙散）、H3（甘露飲加味）、H4（蠶桑合劑）及H5（清•葉天士方）等五種方劑急性毒性試驗。結果小白鼠經口給予H1~H5各方劑10g/kg在一週內無死亡，顯示該等方劑口服殆無毒性。又H2酒精抽取物經由腹腔注射對於小白鼠之LD50為3.85g/kg；其水抽取物經由腹腔注射對於小白鼠之LD50為5.80g/kg；顯示水抽取物之毒性低於酒精抽取物。本研究又探討H1~H5等五種方劑對於實驗性高血脂大白鼠之影響。以血清中膽固醇、三甘油脂、高密度脂蛋白、SGOT及SGPT等濃度之變化為作用之指標，俾進一步暸解其對於肝功能之影響。實驗結果僅H3或H5能降低高血脂大白鼠血清中膽固醇，提升高密度脂蛋白及降低SGOT濃度。由以上結果顯示：H3或H5可能阻斷膽固醇及三甘油脂帶回肝臟代謝排泄，使罹患冠狀動脈硬化之機會減少，及使肝功能趨於正常。本研究再探討丹梔逍遙散封於實驗性肝炎大白鼠進行藥理學實驗，獲致結果：丹梔逍遙散酒精或水抽取物對於四氯化碳誘發大白鼠之急性肝炎所引起血清GOT、GPT、鹼性磷酸酶、總膽紅素等之升高現象，具有顯著降低作用。丹梔是逍遙散酒精或水抽取物對於四氣化碳誘發大白鼠之亞急性肝炎所引起血清GOT之升高現象，具有降低作用。丹梔逍遙散酒精或水抽取物對於實驗性肝炎之改善作用，以水抽取物較酒精抽取物效果顯著。丹梔逍遙散酒精或水抽取物對於實驗性肝炎之改善作用，以誘發肝炎之前就給予丹梔逍遙散者較誘發肝炎之後再給予丹梔逍遙散者效采顯著。

H1 (Chih-Shih-Zhi-Szu-Tang), H2 (Dan-Zhi-Shiau-Yau-San), H3 (Kan-Lu Yin Chia-Wei), H4 (mixed Tsan-Sun prescription) and H5 (Yeh-Tien-Shih prescription) are five traditional Chinese prescriptions usually used to cure hepatitis patient in the China Medical College Hospital. In the present study, we investigated the acute toxicity and the effects of the five Chinese prescriptions in the hyperlipidemia and carbon tetrachloride (CCl4)-induced acute or subacute hepatic damage in rats. Firstly, the five Chinese prescriptions orally at 10g/kg did not induce death in mice. Secondly, only H3 and H5 among the five Chinese prescriptions decreased the plasma cholesterol and SGOT levels, and increased the high density lipoprotein (HDL) levels in the hyperlipidemic rats. Therefore, H3 and H5 might reduced the happened frequency of atherosclerosis via the blockade of cholesterol and triglyceride absorption in the enterointestinal tract and the metabolism in liver. Finally, T.S.(subscript Alc) and T.S.(subscript H2O) could decrease the plasma GOT, GPT, alkaline phosphatase and total bilirubin increased by CCl4 in rats. Therefore, H2 improved the CCl4-induced acute or subacute liver damage in rats, and the effects of T.S.(subscript H2O) were greater than those of Then, the improving effects of T.S.(subscript Alc) and T.S.(subscript H2O) administered before CCl4-induccd acute or subacute liver damage on CCl4-induced acute or subacute liver damage are greater than those of T.S.(subscript Alc) and T.S.(subscript H2O) administered after CCl4-induced acute or subacute liver damage.