健脾方劑對人類精子活動力的體外實驗

The Effect of Spleen-Fortifying Formulas on Human Sperm Motility in Vitro

男性不孕症中，因精液異常所引起的約佔70%~80%，其中精子活動力低為最常見，依中醫理論多屬腎虛或脾虛證。本實驗以燥濕化痰清熱為主的健脾方劑，包括六君子湯、蒼莎導痰湯和二妙散，來探討對人類精子活動力的體外刺激效應。精液樣本分正常組21例，每例精子活動力大於50%，和異常組30例，每例精子活動力小於50%，兩組精子濃度每例每毫升均大於兩千萬隻，合乎世界衛生組織所定的標準。實驗方法，分正常組與異常組精液，每組分加中藥組與僅加F-10溶液對照組，中藥組分三種濃度1.0、0.1以及0.01mg/ml。實驗步驟，每隔60分鐘和120分鐘各於試管中取出10ul，置入精蟲計數器，經由電腦自動精液分析儀(HTM-2030)分析精蟲的活動力。實驗結果，三種健脾方劑無論在60分鐘或120分鐘，對「異常組」的「活動力」和「前進活動力」都有良好的刺激作用，達到統計意義，其機理有待進一步研究。

Abnormalities of semen account for 70-80% of all important cases, and low sperm mobility is a common cause according to tradional Chinese Medicinal theories, patients suffering from low sperm mobility are found mostly to be suffering from kidney or spleen vacuity. In this study, we investigated the effects of ”spleen-fortifying formuals” that ”dry dampness, digest phlegm, and clear heat,” including Liu Jun Zi Tang, Gang Sha Dao Tan Tang and Er Miao San on sperm mobility in vitro. Semen samples from 21 normal patients with sperm motility over 50% or above, and 30 abnormal patients with sperm motility of less than 50% were used. The sperm count in both groups was over 20×10^6, in accordance with World Health Organizations standards. Sperm samples taken from the normal and abnormal control groups were treated with Chinese medical formulas and and F-10 solution respectively. 3 concentrations of 1.0, 0.1 and 0.002 mg/ml respectively were used. At 60 and 120 minutes intervals, 10 μl was removed and placed in a MAKLER counter chamber, and the sperm motility was calculated by computer-assisted analysis (HTM-2030). The results showed that the three formulas markedly stimulated both ”sperm motility” amid ”progressive sperm motility” at 60 minutes and 120 minutes in the abnormal group. The mechanism of this effect requires further investigation.