毛茄及臺灣懸鉤子之美白活性探討

The study of ＂Rubus formosensis＂ and ＂Solanum lasiocarpum＂ on Skin-Whitening Activity

大多數的美白成分都是藉由抑制酪胺酸酶的活性，以抑制黑色素的生合成，且大多為抗氧化物質；並且，抗氧化物質可捕捉因紫外線照射所形成的自由基，直接或間接的抑制由自由基傳遞的二次訊息，因而抑制刺激黑色素生合成的訊息傳遞。台灣懸鉤子地上部及毛茄葉部的水層和酒精層萃取物皆富含多酚類化合物，並且具有良好的抗氧化能力。因此，本研究想探討台灣懸鉤子地上部及毛茄葉部的水層和酒精層萃取物對於黑色素生合成的影響。結果發現台灣懸鉤子及毛茄的萃取物均可抑制體外的酪胺酸酶活性，但台灣懸鉤子萃取物對B16小鼠黑色素瘤細胞具有較強的細胞毒性，而毛茄萃取物則可抑制細胞內的酪胺酸酶活性及黑色素含量。因此，毛茄葉部萃取物可能有潛力發展成為美白產品。

Most whitening ingredients are by inhibiting the activity of tyrosinase, then to inhibit the biosynthesis of melanin, and most of they are antioxidants. Furthermore, antioxidants can capture the free radicals from ultraviolet irradiation, therefore decreasing the radical-induced secondary message, thereby inhibiting the stimulation of melanogenesis, either directly or indirectly. The water and alcohol extracts of ＂Rubus formosensis＂ and ＂Solanum lasiocarpum＂ are rich of polyphenolic compounds, and have good antioxidant capacity. Therefore, the objective of our study is to investigate the water and alcohol extracts of ＂Rubus formosensis＂ and ＂Solanum lasiocarpum＂ on melanogenesis. The results showed that extracts of ＂Rubus formosensis＂ and ＂Solanum lasiocarpum＂ can inhibit tyrosinase activity ＂in vitro＂. The extracts of ＂Rubus formosensis＂ have stronger cytotoxic for B16 mouse melanoma cells. However, extracts of ＂Solanum lasiocarpum＂ can inhibit the intracellular tyrosinase activity and decrease the melanin content. Therefore, ＂Solanum lasiocarpum＂ extract may have the potential to develop into a whitening product.