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篇名 |
移動相中添加β-環糊精在逆相液相層析上分離一些單取代酚類位置異構物
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並列篇名 |
Separation of Some Isomeric Monosubstituted Phenols by RPLC Using β-cyclodextrin as Mobile Phase Additives |
作者 |
李冠儀、張書文、賴鈺森、林振興 |
中文摘要 |
本研究在移動相中添加5 mM β-環糊精於甲醇與水的混合溶液,利用RP-C_(18)靜相管柱分離一些單取代酚類位置異構物,並探討移動相中甲醇含量的影響。由層析結果顯示甲醇與水比例,會顯著影響單取代酚類位置異構物的分離效果。添加5 mM β-環糊精於適當比例的甲醇與水的混合溶液當移動相,利用RP-C_(18)靜相管柱可以有效分離硝基酚、甲酚、乙酚以及萘酚位置異構物,且硝基酚與乙酚可以達到基線分離。 |
英文摘要 |
The retention behavior of some isomeric mono-substituted phenols was investigated by high-performance liquid chromatography on a RP-C_(18) stationary phase using β-cyclodextrin as mobile phase additives. Effects of methanol content of mobile phase were examined. Effective separations of some isomeric mono-substituted phenols were achieved on a RP-C_(18) stationary phase using β-cyclodextrin as mobile phase additives. Moreover, baseline separations of isomeric nitrophenol and ethylphenol were achieved. |
起訖頁 |
56-62 |
關鍵詞 |
逆相液相層析、Reversed phase liquid chromatography、β-環糊精、β-cyclodextrin、硝基酚、Nitrophenol、甲酚、Cresol、乙酚、Ethylphenol、代酚、Naphthol |
刊名 |
嘉南學報:科技類 |
期數 |
201312 (39期) |
出版單位 |
嘉南藥理大學
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