傳統米食對DSS-誘發小鼠結腸發炎病程之影響

The Effect of Traditional Grains on the Pathogenesis of DSS-induced Colitis in Mice

利由DSS（dextran sulfate sodium）誘發結腸炎之小鼠模式，探討紅糯米和黑珍米是否具有緩和病程發展的效果。6~8週大的C57BL/6品系雄鼠，分成4組（1）AIN-93G組、（2）AIN-93G+DSS組、（3）黑珍米＋DSS組、（4）紅糯米＋DSS組，米食添加量約為80%取代基礎飼料的碳水化合物。飼養2週後，除了AIN-93G組外，其餘3組飲水以2% DSS（w/v）取代，於第6天換回一般飲水，DSS誘發腸炎期間觀察並計算疾病活動指數（disease activity index, DAI）。結果顯示DSS刺激後第2天開始，小鼠體重有持續下降的趨勢，由此得知急性大腸炎對小鼠體重有決定性的影響。AIN-93G控制組給予DSS水第2天即開始出現軟便及血便現象，DAI指數快速攀升，顯著高於紅糯米與黑珍米組，存活率以第7天結果得知紅糯米＞黑珍米組＞AIN-93G組，未給予DSS的控制組生長正常、全數存活。結論：DSS誘發腸炎使小鼠體重顯著降低，餵食黑珍米、紅糯米在此腸炎模式中可顯著降低DAI指數，可能具有保護效應。

The aim of this study was to investigate the ameliorative effect of traditional grains on the pathogenesis of dextran sulfate sodium （DSS）-induced colitis in mice. 6-wks-old male C57BL/6 mice were assigned to 4 groups, （1） AIN-93G, （2） AIN-93G+DSS, （3） Hei-Jan rice+DSS and （4） red glutinous rice+DSS. The additional dose of grains were about 80%. After 2-wks feeding, mice were induced colitis by receiving 2% DSS （w/v） in their drinking water for 5 day. During the acute colitis, clinical manifestations and disease activity index （DAI） were recorded everyday. Our results showed that mice treated with DSS had significantly weight loss from day 2. The mice fed AIN-93G-DSS had sever clinical symptoms, marked significantly by diarrhea, gross bleeding and increasing DAI score from day 2. The result of survival rate at day 7 showed red glutinous rice ＞ Hei-Jan rice ＞ AIN-93G in DSS-treated mice. The untreated DSS-mice had normal growth rate and all survival. In summary, body weight loss was observed significantly from day 2 in DSS-induced colitis mice. Mice fed traditional grains had lower DAI score. We suggest traditional grains have ameliorative effect on the pathogenesis of DSS-induced colitis in mice.