In the era of rapidly advancing medical technology, the matter remains unchanged is people’s demand for high-quality care. The practice of evidence-based care is one of the ways to improve the quality of nursing care. This article outlines the evolution of evidence-based nursing, elaborates the role and impediment to the application of evidence-based nursing by nurse practitioners, and the revolution of professional nurse practitioners’ education was discussed. At the end of the article, two actual clinical cases were presented. Through the case studies, we expect that nurse practitioners can understand the application of evidence-based nursing in clinical settings and further to recognize and devote themselves to practicing evidence-based nursing, for improving the quality of care and strengthening the role of nurse practitioners in the health care system