Introduction: Despite the increased awareness of needle sticks and sharps inju-ries and the development of safety needles, these injuries continue to occur not infre-quently in a hospital setting. The purpose of the study was to critically examine these incidences and to identify risk factors associated with them. Methods: We analyzed sharps injuries occurred between 2016 and 2020 in a tertiary medical center in Taiwan. We compared those that occurred in the operating rooms (OR) with those that oc-curred outside the OR. The demographics of the health care workers injured, the of-fending objects, and the mechanisms of injury were compared between two groups of patients. Significant level was determined when p<0.05. Results: Among 157 hospital workers injured during the study period, the group of workers most commonly injured was nurses (56.0%), followed by physicians and surgeons (37.6%). The majority of those injured had a working experience of fewer than three years (73.2%, 115/157). Injuries that occurred outside the OR were self-inflicted due to the inappropriate han-dling of needled syringes. In comparison, 21 (41.2%, 21/51) of injuries in the OR were inflicted by operating surgeons. Among them, 15 (71.4%) were due to inappropriate transfer of surgical sharps. Conclusions: Most sharps injuries that occurred in the OR were due to human factors and were preventable. Greater effort is needed to create a safer working environment in the OR through education of hospital personnel, development of stringent OR policies for handing of surgical instruments, and critical review of every incidence of injury.