This study aims to explore the effect of electro-acustimulation (EA) on attention networks. Sixty participants were randomly divided into EA and sham-EA group. The acupoints LI-4 (HeGu) and PC-6 (NeiGuan) were stimulated by transcutaneous electrical nerve stimulator. Attention network test (ANT) was employed at pre-test, test and post-test phases. Mixed two-factor ANOVA was conducted. Results showed no significant difference between EA and sham-EA in ANT. The alerting function within group at three phases showed statistically significant differences. The level of alerting function at pre-test was lower than during and post-test phases in EA while it was lower than during-test phases only in sham-EA. The non-significant difference between EA and sham-EA in ANT might be due to insufficient doses of stimulation. Whether the enhancement of alerting function in EA comes from the specificity of acupoint selection or electrical stimulation effect requires further confirm. The improvement of alerting function at the during-test phase in sham-EA group might be related to the pressing effect on acupoints.