Building on the construal level theory (Trope & Liberman, 2010), this research investigated the impact of familiarity on human spatial perceptions. When the psychological distance is deemed far (vs. near), individuals adopt a higher construal level to execute cognitive operations, and the spatial perceptions could thus be larger. This research used two studies to examine the impact of environmental familiarity. The survey employed in study 1 examined whether first-year university students and senior university students differ in judging the walking distance of particular locations on the campus. The results partially supported the hypotheses, showing that first-year (vs. senior) students perceived a larger space distance between campus locations. A one-factor (familiarity/unfamiliarity) within-subjects experiment was employed in study 2. The results showed that comparing to the high familiar bus stops, participants reported more walking time perceptions for the low familiar bus stops. Overall, this research found that familiarity would affect spatial perceptions.